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| Registros recuperados: 11 | |
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| Sanchez,Irma Ximena Barbosa; Socarras,Teresa de Jesus Oviedo; Silva,Miguel Ladino; Pereira,Nubia Braga; Machado,Rosangela Zacarias; Vasconcelos,Anilton Cesar. |
| A Erliquiose canina é uma zoonose causada pela Ehrlichia canis, bactéria Gram negativa de distribuição mundial. Alguns cães com erliquiose se tornam portadores assintomáticos enquanto outros desenvolvem uma doença aguda com morte rápida. A apoptose pode ser importante na eliminação de patógenos intracelulares, podendo, nas infecções por Ehrlichia sp. e Anaplasma sp., ocorrer modulação da apoptose celular para prolongar a sobrevivência desses organismos. Para avaliação do papel da apoptose na erliquiose canina, sete cães foram distribuídos em dois grupos. No Grupo inoculado, realizou-se a infecção por via intravenosa com sangue infectado com E. canis (isolado Jaboticabal), sendo realizada a inoculação com PBS estéril nos animais pertencentes ao Grupo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptose; Ehrlichia canis; N-PCR; RIFI; TUNEL; Bcl-2; Bax. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800021 |
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| Nakaghi,Andréa Cristina Higa; Machado,Rosangela Zacarias; Costa,Mirela Tinucci; André,Marcos Rogério; Baldani,Cristiane Divan. |
| The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ehrlichia canis; Dog; IFAT; Dot-ELISA; Nested PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300027 |
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| Moreira,Simone Magela; Machado,Rosângela; Passos,Lygia Friche. |
| The present work describes the detection of infected cells in the bone marrow aspirates of dogs experimentally infected with a Brazilian isolate of Ehrlichia canis. Dogs were monitored twice a day by clinical evaluation and peripheral blood smear examination. Every three days, blood samples were collected for cell counts. Weekly, aspirates from the bone marrow were examined and serum samples were tested by IFAT. The clinical signs observed were fever, pallid membranes, lymphadenopathy, serous nasal secretions, and pronounced weight loss. Hematological alterations included normocytic normochromic anemia, decrease of neutrophils and lymphocytes, and thrombocytopenia. Few E. canis infected cells were seen in blood smears. However, stages of E. canis were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ehrlichia canis; Diagnosis; Bone marrow puncture; Dogs. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000400038 |
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| Aguiar,Daniel Moura; Saito,Taís Berelli; Hagiwara,Mitika Kuribayashi; Machado,Rosângela Zacarias; Labruna,Marcelo Bahia. |
| O presente trabalho relata o isolamento de Ehrlichia canis em cultivo de células DH82 e posterior padronização da Reação de Imunofluorescência Indireta (RIFI). Leucócitos de uma cadela experimentalmente infectada com o isolado Jaboticabal de E. canis foram inoculados em cultivo de células DH82. A inoculação foi monitorada após a segunda semana, a cada 5-6 dias, através de exames citológicos e pela amplificação de um fragmento do gene dsb de Ehrlichia pela Reação em Cadeia pela Polimerase (PCR) para confirmação da infecção. A cultura apresentou-se positiva aos 27 dias pós-inoculação pela PCR e aos 28 dias pela citologia. No 33o dia pós-inoculação, observou-se 20% de células infectadas e, aos 53 dias, 60% de infecção. Atualmente, o isolado encontra-se... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ehrlichia canis; Isolamento; DH82; Imunofluorescência; Sorologia. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300030 |
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| Ferreira, Gabriel Bento; Filippi, Maurício Gianfrancesco; Paes, Antônio Carlos; Lourenço, Maria Lúcia Gomes. |
| The contagious diseases are the most prevalent in Brazilian domestic canines, and one of the most severe is the monocytic ehrlichiosis, caused by Ehrlichia canis bacteria. Among the symptoms of the disease, the most prevalent are apathy, anorexia, epistaxis, hemorrhage, anemia, and thrombocytopenia. However, recent studies indicate association between the disease and diverse symptoms related to cardiac injuries. This article evaluated dogs undoubtedly infected by E. canis through electrocardiogram applied before and after the standard treatment with doxycycline (10mg/kg), aiming to identify typical variations caused by the disease. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monocitic ehrlichiosis; Ehrlichia canis; Electrocardiogram erliquiose monocítica; Ehrlichia canis; Eletrocardiograma. |
| Ano: 2017 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/37335 |
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| Aguiar,Daniel M.; Hagiwara,Mitika K.; Labruna,Marcelo B.. |
| An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ehrlichia canis; Dog; Molecular Characterization; Ehrlichiosis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014 |
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| Oliveira,L.P.; Cardozo,G.P.; Santos,E.V.; Mansur,M.A.B.; Donini,I.A.N.; Zissou,V.G.; Roberto,P.G.; Marins,M. |
| The partial DNA sequences of the 18S rRNA gene of Babesia canis and the 16S rRNA gene of Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil, were compared to sequences from other strains deposited in GenBank. The E. canis strain circulating in Ribeirão Preto is identical to other strains previously detected in the region, whereas the subspecies Babesia canis vogeli is the main Babesia strain circulating in dogs from Ribeirão Preto. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Babesia canis vogeli; Ehrlichia canis; Ticks; RRNA gene; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200006 |
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| Moreira,S.M.; Bastos,C.V.; Araújo,R.B.; Santos,M.; Passos,L.M.F.. |
| The present work describes a retrospective study of clinical cases of ehrlichiosis in dogs examined from March 1998 to September 2001. From the clinical records with laboratorial confirmation of Ehrlichia canis or E. platys infections, the following parameters were analyzed: demographic aspects (age, race, sex, period of the year and origin), clinical characteristics (body temperature, exposure to ticks and clinical signs), and hematological characteristics (blood cell counts and type of infected cell). A total of 194 clinical records were analyzed, from which 31 animals were infected with E. canis and 21 animals with E. platys. The number of cases of canine ehrlichiosis increased considerably from the year 2000 onwards, and 24.4% of the cases occurred in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Ehrlichia canis; Ehrlichia platys; Epidemiology. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000200003 |
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| 坂田, 義美; 市川, 康明; 猪熊, 壽; SAKATA, Yoshimi; ICHIKAWA, Yasuaki; INOKUMA, Hisashi. |
| 犬のE.canisおよびA.ρhagocytophilum感染状況を明らかにするため，従来法である蛍光抗体法(IFA)，および組換え主要膜表面蛋白質を抗原とした酵素抗体法を原理とする新規の特異抗体検出キットを組合せて全国的血清疫学調査を行った. IFAによるスクリーニング検査では1,206検体中64頭(5.3%)がE.canis 20倍以上の抗体価を示したが，これら64頭は特異抗体検出キットではすべてE.canis陰性であり，E. canis以外の近縁種に感染していたと考えられた.いっぽうIFAでA.ρhagocytophilumに対して20倍以上の抗体価を示したものは1,206検体中3頭(0.2%)であった.うち1頭は特異抗体検出キットでもA.phagocytophilum陽性を示し，日本にもA.phagocytophilumに感染した犬 がいることが示唆された.しかし，抗体陽性犬に臨床症状は認められず，病原性との関連は不明であった. A seroepidemiological survey of Ehrlichia canis and Anaplasma phagocytophilum infection in dogs in Japan was performed by using a combination of a classical immunofluorescence antibody assay (IFA) and a new enzyme immune assay (EIA) kit that uses a specific recombinant major surface protein of the pathogens. Although 64 (5.3%) of 1,206... |
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Palavras-chave: 犬; エールリッヒア; アナプラズマ; 血清疫学調査; Anaplasma phagocytophilum; Dog; Ehrlichia canis. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2818 |
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| Registros recuperados: 11 | |
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